Neurogenic differentiation of human dental stem cells in vitro
이주희, Um So-Youn, 송인석, 김희영, 서병무,
소속 상세정보
이주희 ( Lee Joo-Hee ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
( Um So-Youn ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
송인석 ( Song In-Seok ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
김희영 ( Kim Hui-Young ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
서병무 ( Seo Byoung-Moo ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
KMID : 0355620140400040173
Abstract
Objectives: The purpose of this study was to investigate the neurogenic differentiation of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and stem cells from apical papilla (SCAP).
Materials and Methods: After induction of neurogenic differentiation using commercial differentiation medium, expression levels of neural markers, microtubule-associated protein 2 (MAP2), class III β-tubulin, and glial fibrillary acidic protein (GFAP) were identified using reverse transcriptase polymerase chain reaction (PCR), real-time PCR, and immunocytochemistry.
Results: The induced cells showed neuron-like morphologies, similar to axons, dendrites, and perikaryons, which are composed of neurons in DPSCs, PDLSCs, and SCAP. The mRNA levels of neuronal markers tended to increase in differentiated cells. The expression of MAP2 and β-tubulin III also increased at the protein level in differentiation groups, even though GFAP was not detected via immunocytochemistry.
Conclusion: Human dental stem cells including DPSCs, PDLSCs, and SCAP may have neurogenic differentiation capability in vitro. The presented data support the use of human dental stem cells as a possible alternative source of stem cells for therapeutic utility in the treatment of neurological diseases.
키워드
Dental pulp; Periodontal ligament; Dental papilla; Stem cells; Neurogenic differentiation
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